Electron Microscopy (EM) and Cryo-EM - Practical Course

28-29 October & 4-5 November 2024

Dr. Christel Genoud, UNIL

Jean Daraspe, UNIL

Antonio Mucciolo, UNIL

Damien De Bellis, UNIL

2 conférencier·ères externes

Mornings: lectures

Afternoons: hands-on demonstrations + work on participant’s samples

2 days focusing on classic EM,

2 days on Cryo EM

Goals:
-identify the best EM method for each student samples/questions;
-discover which EM techniques that are available at the EMF could forward your research;
-identify how molecules can be localized in cells or tissues;
-learn the basics of EM image acquisition, analysis and reconstruction;
-understand basic SEM and TEM operating principles;
-gain exposure to various methods of preparing biological samples for EM together with the advantages and disadvantages related to each of these methods

Topics covered:
TEM, transmission electron microscopy;
cryoTEM;
TEM sample preparation: chemical and high-pressure freezing procedures;
SEM, scanning electron microscopy;
SEM sample preparation;
Volume EM: TEM- tomography, Focused Ion Beam-SEM, Array Tomography;
Ultramicrotomy, resin sections;
Cryo-ultramicrotomy, the Tokuyasu technique;
Image processing, volume reconstruction, and modelling

> On the first day of activity, each student has 3 minutes (1-2 slides) to explain his needs in electron microscopy in the frame of the PhD work.

More Information will follow

UNIL, room TBA

Reimbursements for CUSO StarOmics students: Train ticket, 2°class, half-fare from your institution to the place of the activity.

Reimbursement of your travel tickets can be asked online through your MyCUSO.

For any question concerning reimbursement please contact the CUSO StarOmics coordinator
Other CUSO Students: Please contact the coordinator of your Program.

Registation is CLOSE

Deadline for registration: TBA

12 places available for CUSO students (4 for each participating program)

For cancellations after the deadline of registration or no show: 150 CHF administrative fees

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